GC-MS-based metabolomics study of the responses to arachidonic acid in Blakeslea trispora

The potential use of arachidonic acid (AA) to enhance the production of β-carotene in Blakeslea trispora was investigated in this work. To study the mechanism of the B. trispora response to AA, we used a systematic analytical approach to investigate the changes in the B. trispora cell metabolome at different time points after AA treatment. A maximum of β-carotene production was obtained when 0.4g/l AA was added after 36h of cultivation. Gas chromatography-mass spectrometry (GC-MS)-based metabolomics approach and a multivariate analysis were used to investigate the intracellular biochemical changes in B. trispora. With the aid of principal component analysis (PCA), the intracellular metabolite profiles of all the groups were distinguished. Moreover, a group classification and pairwise discrimination between the control and AA-treated groups were obtained through partial least-squares-discriminant analysis (PLS-DA), and 27 differential metabolites with variable importance in the projection (VIP) value higher than 1 were identified, which was also confirmed by the subsequent hierarchical cluster analysis (HCA). Separation of the control and AA-treated groups was mainly due to the compounds of the Krebs cycle, fatty acids and amino acids. With the treatment of AA, the glycolysis was enhanced and the use of glucose for fermentation was increased. The increased levels of some fatty acids and decreased levels of amino acids in the AA-treated cells could also be the responses to the addition of AA. Metabolomics provided a powerful methodology to gain insight in metabolic changes induced by metabolic stimulators in microorganisms.