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Characterization of γ-glutamyl kinase mutants from Saccharomyces cerevisiae
Yuki Tatehashi1, Hiroshi Takagi
1Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
Journal of Bioscience and Bioengineering
|June 18, 2013
Abstract:
We characterized the yeast γ-glutamyl kinase important for proline biosynthesis. Asp154 and Ile150 were involved in the affinity with glutamate as well as proline. The Asp154Asn and Ile150Thr mutants had increased thermostability, in addition to desensitization to proline. Deletion of a C-terminal domain dramatically decreased the reaction rate and stability.
