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Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae
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Protein trafficking and maturation regulate intramembrane proteolysis.

Yuichi Morohashi1, Taisuke Tomita

  • 1Department of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan.

Biochimica Et Biophysica Acta
|June 18, 2013
PubMed
Summary

Intramembrane-cleaving proteases (I-CLiPs) regulate cellular signaling and disease by cleaving membrane proteins. Their activity is finely tuned by intracellular vesicle trafficking, influencing substrate encounters and cleavage site preference.

Keywords:
Intramembrane proteolysisMembrane proteinVesicle trafficking

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Background:

  • Intramembrane-cleaving proteases (I-CLiPs) are crucial membrane-embedded enzymes.
  • Their substrates are membrane proteins involved in cellular signaling and human diseases.
  • Proper localization of I-CLiPs and substrates is essential for regulated proteolysis.

Purpose of the Study:

  • To review recent advances in understanding I-CLiP regulation.
  • To highlight the role of intracellular vesicle trafficking in I-CLiP activity.
  • To discuss how trafficking influences substrate recognition and cleavage site specificity.

Main Methods:

  • Literature review of studies on I-CLiPs and membrane trafficking.
  • Analysis of mechanisms regulating I-CLiP-substrate interactions.
  • Discussion of the impact of intracellular transport on intramembrane proteolysis.

Main Results:

  • Intracellular membrane trafficking plays a critical role in controlling I-CLiP function.
  • Trafficking pathways limit non-specific interactions between I-CLiPs and substrates.
  • Vesicle transport influences the precise cleavage site selection by I-CLiPs.

Conclusions:

  • Intramembrane proteolysis by I-CLiPs is tightly regulated by intracellular vesicle trafficking.
  • Understanding these trafficking mechanisms is key to deciphering I-CLiP roles in signaling and disease.
  • This review synthesizes current knowledge on trafficking-mediated regulation of I-CLiPs.