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C2ORF40 suppresses breast cancer cell proliferation and invasion through modulating expression of M phase cell cycle

Jing Lu1, Mingxin Wen, Yurong Huang

  • 1Department of Anatomy and Key Laboratory of Experimental Teratology; Ministry of Education; Shandong University School of Medicine; Jinan, Shandong, P.R. China.

Epigenetics
|June 18, 2013
PubMed

Insights

C2ORF40 acts as a tumor suppressor in breast cancer by silencing gene expression via promoter hypermethylation. Its reduced levels correlate with poor prognosis, inhibiting cancer cell growth and metastasis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • C2ORF40 is a potential tumor suppressor in breast cancer.
  • The mechanisms of C2ORF40 downregulation and its precise function in breast cancer are not fully understood.

Purpose of the Study:

  • To investigate the mechanism of C2ORF40 silencing in breast cancer.
  • To elucidate the functional role of C2ORF40 in breast cancer progression.
  • To evaluate C2ORF40 as a prognostic marker.

Main Methods:

  • Analysis of C2ORF40 expression and promoter methylation in human breast cancers and cell lines.
  • Functional studies involving C2ORF40 overexpression and silencing in breast cancer cells.
  • Bioinformatics and Fluorescence-Activated Cell Sorting (FACS) analysis.

Main Results:

  • C2ORF40 is frequently silenced in breast cancers due to promoter hypermethylation.
  • Lower C2ORF40 mRNA levels are linked to reduced disease-free survival and increased distant metastasis.
  • C2ORF40 overexpression inhibits cell proliferation, migration, and invasion, while its silencing promotes these phenotypes.
  • C2ORF40 regulates cell cycle at the G2/M phase by downregulating mitotic genes like UBE2C.

Conclusions:

  • C2ORF40 functions as a tumor suppressor in breast cancer development and progression.
  • C2ORF40 is a potential prognostic biomarker for breast cancer patients.
Keywords:
C2ORF40DNA methylationbreast cancermetastasismitosisproliferation

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