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Updated: May 10, 2026

Respirometric Oxidative Phosphorylation Assessment in Saponin-permeabilized Cardiac Fibers
Published on: February 28, 2011
Relationship between cardiolipin metabolism and oxygen availability in Bacillus subtilis
Simona Lobasso1, Luigi L Palese, Roberto Angelini
1Department of Basic Medical Sciences, Neuroscience and Sensory Organs, University of Bari "Aldo Moro", Italy.
Abstract:
We report changes of the content of anionic phospholipids in Bacillus subtilis in response to hypoxic conditions and inhibition of terminal respiration. Cardiolipin accumulates rapidly when bacteria are suspended in non-growth medium under reduced aeration or exposed to the inhibitor cyanide; the increase of cardiolipin occurs at the expense of its precursor phosphatidylglycerol and is temperature-dependent. Depending on the extent of hypoxic stress, membranes containing different levels of cardiolipin can be isolated from B. subtilis cells. The NADH oxidase activity in cardiolipin-enriched membranes is cyanide-resistant; furthermore O2 consumption measurements indicated that cardiolipin-enriched cells are resistant to cyanide. Results point out a possible interdependence between the effect of cyanide on cardiolipin metabolism and the effect of cardiolipin on the effectiveness of cyanide inhibition.
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