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Nucleosome structural changes induced by binding of non-histone chromosomal proteins HMGN1 and HMGN2

Hideto Shimahara1, Takaaki Hirano, Kouichi Ohya

  • 1Center for Nano Materials and Technology, Japan Advanced Institute of Science and Technology (JAIST), 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.

FEBS Open Bio
|June 18, 2013
PubMed

Keywords:
CDCD, circular dichroismHMG, high mobility groupHMGNHMGN1 HMGN2, non-histone chromosomal proteinsIPTG, isopropyl-β-d-galactopyranosideLB, Luria–BertaniMNase, micrococcal nucleaseNMR, nuclear magnetic resonanceNucleosomePCR, polymerase chain reactionRP-HPLC, reverse phase high performance liquid chromatographyReconstitutionSDS–PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresisUnmodified recombinant histonesphH2A, phH2B, phH3, and phH4, vectors for the gene expression of all four recombinant human core histones H2A, H2B, H3, and H4, respectively

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