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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

Updated: May 10, 2026

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
09:30

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

The Gray Institute 'open' high-content, fluorescence lifetime microscopes.

P R Barber1, I D C Tullis, G P Pierce

  • 1Gray Institute for Radiation Oncology & Biology, Dept. Of Oncology, University of Oxford, Oxford, UK. paul.barber@oncology.ox.ac.uk

Journal of Microscopy
|June 19, 2013
PubMed
Summary
This summary is machine-generated.

We developed an open-source microscopy platform for advanced fluorescence imaging, enabling easier adoption of techniques like time-domain fluorescence imaging in biology. This modular design enhances flexibility and automation for high-content screening applications.

Keywords:
FLIMFRETTCSPChigh-content microscopytissue microarray

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Last Updated: May 10, 2026

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
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Fluorescence Lifetime Macro Imager for Biomedical Applications
06:01

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

Area of Science:

  • Biophotonics and advanced microscopy techniques.
  • Development of modular and open-source scientific instrumentation.

Background:

  • Traditional microscopy platforms can be inflexible and costly, hindering the adoption of advanced techniques.
  • Transitioning novel microscopy methods from research to routine biological applications requires adaptable and accessible instrumentation.

Purpose of the Study:

  • To present an open design methodology for constructing flexible, automated microscopy platforms.
  • To demonstrate the first implementation of time-domain fluorescence microscopy on such a platform.
  • To facilitate the integration of advanced imaging techniques into routine biological research.

Main Methods:

  • Developed a modular microscopy design using commercial off-the-shelf and custom components.
  • Implemented time-domain fluorescence imaging (FLIM) using time-correlated single photon counting.
  • Engineered automated high-content fluorescence microscope implementations for multiwell plates and tissue microarrays.

Main Results:

  • Successfully constructed several automated high-content fluorescence microscope platforms.
  • Demonstrated automated wide-field and laser scanning capabilities for high-content microscopy.
  • Integrated time-domain FLIM into an automated, flexible platform.

Conclusions:

  • The open-design modular microscopy approach enables flexible, expandable, and automated advanced imaging.
  • This methodology facilitates the transition of techniques like time-domain fluorescence microscopy to routine biological applications.
  • The versatile design is extendable for various applications, including use as radiation-beam end-stations.