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High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii
Published on: December 5, 2016
A newly isolated Chlorella sp. from desert sand crusts exhibits a unique resistance to excess light intensity
Haim Treves1, Hagai Raanan, Omri M Finkel
1Department of Plant and Environmental Sciences, Edmond J. Safra Campus - Givat Ram, The Hebrew University of Jerusalem, Jerusalem, Israel.
Abstract:
We recently isolated a small green alga from a biological sand crust (BSC) in the NW Negev, Israel. Based on its 18S rRNA and rbcL genes, it is a close relative of Chlorella sorokiniana and of certain strains of C. vulgaris and C. variabilis, but differs substantially in many aspects from C. sorokiniana. Because the classification of Chlorellales is still not resolved, we designated this species as C. ohadii (Trebouxiophyceae) in honor of Professor Itzhak Ohad. Under controlled laboratory conditions, C. ohadii showed marked structural and photosynthetic performance changes, depending on the carbon source used during growth, as well as remarkable resistance to photoinhibition. CO2 -dependent O2 evolution was not affected even when exposed to a light intensity of 3500 μmole photons m(-2) s(-1) , over 1.5 times the maximal intensity reached at the BSC surface, whereas the variable fluorescence declined sharply. We briefly discuss the use of fluorescence to assess photosynthetic rate and the implications of this finding for the assessment of global BSCs activity.
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