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Related Experiment Video

Updated: May 10, 2026

Intracavernosal Pressure Recording to Evaluate Erectile Function in Rodents

Intracavernosal Pressure Recording to Evaluate Erectile Function in Rodents

Published on: June 6, 2018

Sildenafil citrate improves erectile function after castration in a rat model.

John P Mulhall¹, Nipun Verma, Serkan Deveci

¹Department of Urology, Memorial Sloan-Kettering Cancer Center, New York, USA.

BJU International

|June 19, 2013

Summary

Sildenafil treatment improved erectile function in rats post-castration. This phosphodiesterase 5 inhibitor enhanced erectile function without altering collagen or apoptosis levels in erectile tissue.

Keywords:

castration orchidectomy phosphodiesterase type 5 inhibitor rat sildenafil

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Last Updated: May 10, 2026

Intracavernosal Pressure Recording to Evaluate Erectile Function in Rodents

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Published on: June 6, 2018

Cavernous Nerve Stimulation and Recording of Intracavernous Pressure in a Rat

Cavernous Nerve Stimulation and Recording of Intracavernous Pressure in a Rat

Published on: April 23, 2018

Area of Science:

Urology
Pharmacology
Reproductive Biology

Background:

Castration leads to erectile dysfunction.
Phosphodiesterase 5 inhibitors (PDE5i) are used to treat erectile dysfunction.
The impact of PDE5i on erectile function following castration requires further investigation.

Purpose of the Study:

To evaluate the efficacy of sildenafil citrate in improving erectile function after castration in a rat model.
To determine if sildenafil treatment mitigates collagenization and apoptosis in erectile tissue post-castration.

Main Methods:

Sixty Sprague-Dawley rats underwent sham surgery, castration, or castration plus sildenafil treatment.
Erectile function was assessed by measuring the intracavernosal pressure-to-mean arterial pressure (ICP/MAP) ratio at 7 and 28 days.
Apoptotic indices (AIs) and smooth muscle-collagen (SM-C) ratios were evaluated using TUNEL and Masson's trichrome staining, respectively.

Main Results:

Sildenafil treatment significantly improved ICP/MAP ratios in castrated rats compared to controls at both 7 and 28 days.
Despite improvements, erectile function in sildenafil-treated rats remained below that of sham-operated rats.
No significant differences were observed in SM-C ratios or AIs between the castration-only and sildenafil-treated groups.

Conclusions:

Sildenafil administration effectively improves erectile function in rats following castration.
The mechanism of improved erectile function with sildenafil post-castration does not appear to involve protection against smooth muscle collagenization or reduced apoptosis.
Early intervention with PDE5 inhibitors may be a viable strategy for preserving erectile function after castration.