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High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping
Published on: June 29, 2018
Hybridisation, paternal leakage and mitochondrial DNA linearization in three anomalous fish (Scombridae)
Jess A T Morgan1, Michael Macbeth, Damien Broderick
1Molecular Fisheries Laboratory, University of Queensland, PO Box 6097, St Lucia, Queensland 4072, Australia; Queensland Alliance for Agriculture and Food Innovation, University of Queensland, 306 Carmody Rd, St Lucia, Queensland 4072, Australia.
Abstract:
Using mitochondrial DNA for species identification and population studies assumes that the genome is maternally inherited, circular, located in the cytoplasm and lacks recombination. This study explores the mitochondrial genomes of three anomalous mackerel. Complete mitochondrial genome sequencing plus nuclear microsatellite genotyping of these fish identified them as Scomberomorus munroi (spotted mackerel). Unlike normal S. munroi, these three fish also contained different linear, mitochondrial genomes of Scomberomorus semifasciatus (grey mackerel). The results are best explained by hybridisation, paternal leakage and mitochondrial DNA linearization. This unusual observation may provide an explanation for mtDNA outliers in animal population studies.
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