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Updated: May 10, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
Sheathless capillary electrophoresis electrospray ionization-mass spectrometry interface based on
1Department of Chemistry, National Taiwan University, Taipei, Taiwan.
Abstract:
This study develops a sheathless CE-MS interface using a robust PDMS membrane emitter and liquid-film electric conduction. A 3D mold was constructed for casting the device by using a one-step casting procedure. The interface consisted of a 125 μm-thick triangular emitter with a 50 μm-diameter microchannel, a conducting reservoir, and a 375 μm-diameter channel for assembling the separation capillary. The separation capillary was inserted into the 375 μm channel and connected to the emitter through the conducting reservoir. The electric contact for the CE outlet was established through a conductive liquid film in the space between the capillary terminus and the 375 μm channel. The one-step casting procedure and using a membrane emitter instead of a tapered emitter produced an easily fabricated and robust interface. A stable electrospray was obtained from 30 to 350 nL/min. Analyzing a five-peptide mixture in low-EOF (60 nL/min) and high-EOF (210 nL/min) conditions demonstrated the utility of the interface.
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