Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

Oxygen Requirements and Growth Patterns

Oxygen Requirements and Growth Patterns

Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...

Oxygenic Photosynthesis

Oxygenic Photosynthesis

Oxygenic photosynthesis is a fundamental process in which light energy is harnessed to drive the oxidation of water, leading to the production of molecular oxygen (O₂), adenosine triphosphate (ATP), and nicotinamide adenine dinucleotide phosphate (NADPH). This process is essential for sustaining aerobic life on Earth and is primarily carried out by cyanobacteria, algae, and plants. The core of oxygenic photosynthesis lies in the thylakoid membranes, where chlorophyll pigments facilitate light...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Metabolic flexibility secures skeletal progenitor function upon reduced glycolysis driven by PFKFB3 loss.

Bone·2026

Same author

Hybrid Oxygen-Sensing Bio-Scaffolds for 3D Micro-Tissue Models.

Biosensors·2026

Same author

Correlating Stimulated Emission Depletion Microscopy With Fluorescence Lifetime Imaging Microscopy to Study the TIE2 Protein on Kidney Glomerular Podocytes.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026

Same author

A novel model to study the impact of gender-affirming therapy on bone in young male mice.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·2026

Same author

Cell-specific mRNA delivery via nanobody-functionalized lipid nanoparticles.

Journal of controlled release : official journal of the Controlled Release Society·2025

Same author

Visualizing the internalization and biological impact of nanoplastics in live intestinal organoids by Fluorescence Lifetime Imaging Microscopy (FLIM).

Light, science & applications·2025

Search research articles

Related Experiment Video

Updated: May 10, 2026

Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

Luminescence Lifetime Imaging of O₂ with a Frequency-Domain-Based Camera System

Published on: December 16, 2019

Biological detection by optical oxygen sensing.

Dmitri B Papkovsky¹, Ruslan I Dmitriev

¹Biochemistry Department, University College Cork, College Road, Cork, Ireland. d.papkovsky@ucc.ie.

Chemical Society Reviews

|June 19, 2013

Summary

Optical oxygen (O2) sensing using quenched-phosphorescence offers sensitive, non-invasive biological detection. This review covers applications from cell respiration to in vivo measurements, highlighting future potential.

More Related Videos

Creating Rapid Oxygen Oscillations in Microbial Single-cell Growth Analysis using a Microfluidic Double-layer Device

Creating Rapid Oxygen Oscillations in Microbial Single-cell Growth Analysis using a Microfluidic Double-layer Device

Published on: July 18, 2025

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Published on: March 3, 2010

Related Experiment Videos

Last Updated: May 10, 2026

Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

Luminescence Lifetime Imaging of O₂ with a Frequency-Domain-Based Camera System

Published on: December 16, 2019

Creating Rapid Oxygen Oscillations in Microbial Single-cell Growth Analysis using a Microfluidic Double-layer Device

Creating Rapid Oxygen Oscillations in Microbial Single-cell Growth Analysis using a Microfluidic Double-layer Device

Published on: July 18, 2025

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Published on: March 3, 2010

Area of Science:

Biomedical Engineering
Analytical Chemistry
Biophysics

Background:

Optical sensing of molecular oxygen (O2) is crucial for biological detection.
Quenched-phosphorescence is a key O2 sensing technique.
Sensor technology involves specific principles, materials, and formats.

Purpose of the Study:

To review recent developments in optical O2 sensing for biological detection.
To emphasize the quenched-phosphorescence O2 sensing technique.
To describe various applications and future prospects.

Main Methods:

Review of scientific literature on optical O2 sensing.
Focus on quenched-phosphorescence principles and sensor technology.
Categorization and discussion of diverse biological applications.

Main Results:

Optical O2 sensing enables minimally invasive, contact-less bioanalysis.
High sensitivity, flexibility, and 2D/3D imaging capabilities are key advantages.
Applications span enzymatic assays, cell respiration, safety, and in vivo monitoring.

Conclusions:

Optical O2 sensing, particularly quenched-phosphorescence, provides versatile bioanalytical tools.
The technology supports novel tasks in various biological models and sample types.
Future developments promise further advancements in sensitivity and application scope.