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Updated: May 10, 2026

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
Published on: September 11, 2017
Mapping the driving forces of chromosome structure and segregation in Escherichia coli
Nathan J Kuwada1, Keith C Cheveralls, Beth Traxler
1Department of Physics and Department of Bioengineering, University of Washington, Seattle, WA 98195, USA, Biophysics Graduate Group, University of California, Berkeley, CA 94720, USA and Department of Microbiology, University of Washington, Seattle, WA 98195, USA.
Abstract:
The mechanism responsible for the accurate partitioning of newly replicated Escherichia coli chromosomes into daughter cells remains a mystery. In this article, we use automated cell cycle imaging to quantitatively analyse the cell cycle dynamics of the origin of replication (oriC) in hundreds of cells. We exploit the natural stochastic fluctuations of the chromosome structure to map both the spatial and temporal dependence of the motional bias segregating the chromosomes. The observed map is most consistent with force generation by an active mechanism, but one that generates much smaller forces than canonical molecular motors, including those driving eukaryotic chromosome segregation.
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