Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
Protein Folding Quality Check in the RER01:29

Protein Folding Quality Check in the RER

ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A High-Quality Genome Sequence of Model Legume <i>Lotus japonicus</i> (MG-20) Provides Insights into the Evolution of Root Nodule Symbiosis.

Genes·2020
Same author

Surface-plasmon-coupled chemiluminescence amplification of silver nanoparticles modified immunosensor for high-throughput ultrasensitive detection of multiple mycotoxins.

Analytica chimica acta·2020
Same author

Neurofibromatosis type 1 due to possible maternal mosaicism in a family with two affected siblings.

Congenital anomalies·2020
Same author

A novel machine learning unsupervised algorithm for sleep/wake identification using actigraphy.

Chronobiology international·2020
Same author

Hematological Characteristics of Hb Constant Spring (<i>HBA2</i>: c.427T>C) Carriers in Mainland China.

Hemoglobin·2020
Same author

Annotation and characterization of Babesia gibsoni apicoplast genome.

Parasites & vectors·2020
Same journal

Epidemiological characteristics of amebiasis in Japan from 2001 to 2022.

PloS one·2026
Same journal

Longitudinal associations of academic stress with eating related patterns, nutrition, somatic indicators, and depressive symptoms in university students: A study protocol.

PloS one·2026
Same journal

Pollution removal efficiency enhancement by agricultural biomass additions in constructed wetlands: A framework integrating meta-analysis with explainable machine learning.

PloS one·2026
Same journal

Insulation failure mapping on power transformer bushing using FRA and electrostatic simulation.

PloS one·2026
Same journal

Enhancing medical Q&A systems with multimodal knowledge graphs and dual-layer attention mechanisms.

PloS one·2026
Same journal

UAMP: Consistent video object segmentation with uncertainty-aware memory propagation.

PloS one·2026
See all related articles

Related Experiment Video

Updated: May 10, 2026

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
07:08

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

A method for WD40 repeat detection and secondary structure prediction.

Yang Wang1, Fan Jiang, Zhu Zhuo

  • 1Lab of Computational Chemistry and Drug Design, Laboratory of Chemical Genomics, Peking University Shenzhen Graduate School, Shenzhen, PR China.

Plos One
|June 19, 2013
PubMed
Summary
This summary is machine-generated.

A new program, WDSP (WD40 repeat protein Structure Predictor), accurately identifies WD40 repeats and predicts their structures. This tool aids in understanding these vital eukaryotic proteins and their roles in complex assembly.

More Related Videos

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

Related Experiment Videos

Last Updated: May 10, 2026

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
07:08

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

Published on: July 14, 2015

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

Area of Science:

  • Structural biology
  • Bioinformatics
  • Proteomics

Background:

  • WD40-repeat proteins (WD40s) are a large eukaryotic family crucial for protein-protein, DNA, and RNA complex assembly.
  • Despite sequence diversity, WD40 proteins share conserved β-propeller structures.
  • Understanding WD40 protein structure is vital for deciphering their biological functions.

Purpose of the Study:

  • To develop a computational tool for accurate identification and secondary structure prediction of WD40 repeats.
  • To address challenges posed by sequence variability and complex domain arrangements in WD40 proteins.
  • To enhance the prediction of multiple WD40-domain proteins.

Main Methods:

  • Development of WDSP (WD40 repeat protein Structure Predictor).
  • Incorporation of local residue information and family-specific, non-local structural features.
  • Consideration of the global combination of repeats for improved multi-domain protein prediction.

Main Results:

  • WDSP accurately identifies WD40 repeats and predicts secondary structures.
  • The method effectively handles highly diversified protein sequences and variable loop regions.
  • Achieved an average Q3 accuracy of 93.7% in secondary structure prediction via jack-knife testing.
  • Demonstrated utility using the disease-related protein LRRK2.

Conclusions:

  • WDSP is an effective tool for WD40 protein structure prediction.
  • The program's design overcomes sequence diversity and loop variability challenges.
  • WDSP facilitates a deeper understanding of WD40 protein structures and functions, with implications for disease-related proteins.