Related Experiment Video
Updated: May 10, 2026
07:31
A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate
Published on: May 3, 2021
Downregulated RhoBTB2 expression contributes to poor outcome in osteosarcoma patients
Zhe Jin1, Ya-Xin Han, Xiao-Rui Han
1Department of Orthopaedics, The First Affiliated Hospital of China Medical University , Shenyang, China .
Cancer Biotherapy & Radiopharmaceuticals
|June 20, 2013
Summary
RhoBTB2 protein is underexpressed in osteosarcoma, correlating with recurrence. Its overexpression induces cell cycle arrest and apoptosis, suggesting RhoBTB2 as a potential therapeutic target for osteosarcoma.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Osteosarcoma is a prevalent malignant bone tumor.
- RhoBTB2 protein plays a role in cell cycle regulation and apoptosis.
- Understanding RhoBTB2's function is crucial for developing osteosarcoma therapies.
Purpose of the Study:
- To investigate RhoBTB2 protein expression in osteosarcoma tissues.
- To analyze the correlation between RhoBTB2 expression and clinicopathological features.
- To determine the functional impact of RhoBTB2 on osteosarcoma cell behavior.
Main Methods:
- Real-time PCR and immunohistochemistry for RhoBTB2 expression analysis in 121 osteosarcoma specimens.
- Chi-square test to correlate RhoBTB2 levels with clinicopathological parameters.
- Transfection of human osteosarcoma (HOS) cells with RhoBTB2 plasmid, followed by MTT assay and flow cytometry.
Main Results:
- RhoBTB2 protein expression was significantly lower in osteosarcoma tissues compared to normal tissues.
- Reduced RhoBTB2 expression was associated with primary tumor location and local recurrence.
- Overexpression of RhoBTB2 in HOS cells led to G1 phase cell cycle arrest and induced apoptosis.
Conclusions:
- RhoBTB2 plays a significant role in osteosarcoma development and progression.
- RhoBTB2 expression levels may serve as a diagnostic and prognostic marker.
- RhoBTB2 represents a promising novel therapeutic target for osteosarcoma treatment.
Related Concept Videos
01:20The Retinoblastoma Gene
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
01:15Receptor Downregulation in MVBs
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR activation may...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR activation may...
03:03mTOR Signaling and Cancer Progression
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
01:03Rous Sarcoma Virus (RSV) and Cancer
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
01:03Rous Sarcoma Virus (RSV) and Cancer
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...