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Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining
Published on: June 23, 2020
Benzothiazoline based chemodosimeters for fluorogenic detection of hypochlorous acid.
Zhisheng Wu1, Xuanjun Wu, Zhu Li
1Department of Chemical Biology, College of Chemistry and Chemical Engineering, and Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen 361005, China.
Bioorganic & Medicinal Chemistry Letters
|June 20, 2013
Summary
New chemodosimeters were created to detect hypochlorous acid (HOCl) using color and fluorescence changes. These nonfluorescent, colorless compounds offer a novel method for HOCl sensing.
Area of Science:
- Analytical Chemistry
- Organic Chemistry
- Biochemistry
Background:
- Hypochlorous acid (HOCl) is a reactive oxygen species crucial in biological processes.
- HOCl plays a role in inflammation and oxidative stress.
- Accurate detection of HOCl is vital for biological and medical research.
Purpose of the Study:
- To design and synthesize novel chemodosimeters for HOCl detection.
- To achieve chromo-fluorogenic sensing of HOCl.
- To develop tools for studying HOCl in biological systems.
Main Methods:
- Synthesis of benzothiazoline-based chemosensors.
- Spectroscopic analysis (UV-Vis and fluorescence) for detection.
- Evaluation of sensor response to HOCl.
Main Results:
- Two nonfluorescent and colorless chemodosimeters were successfully synthesized.
- The chemodosimeters exhibited selective chromo-fluorogenic responses to HOCl.
- The developed probes demonstrated high sensitivity and specificity for HOCl detection.
Conclusions:
- The novel chemodosimeters provide an effective platform for HOCl detection.
- These probes can be utilized for real-time monitoring of HOCl in biological samples.
- The findings contribute to the advancement of analytical tools for reactive oxygen species research.
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