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Bergmeyer Glucose Quantification for Microbiological Samples
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Published on: January 17, 2025

A facile channel for D-glucose detection in aqueous solution.

Zhijun Wang1, Haiying Lei, Liheng Feng

  • 1Department of Chemistry, Changzhi University, Changzhi 046011, PR China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|June 20, 2013
PubMed
Summary

New fluorescent probes using NAHBDS and boronic acid substituted viologens (BBVs) offer sensitive and reversible detection of D-glucose. These ensembles provide a novel approach for designing selective glucose sensing systems.

Keywords:
Boronic acidChemosensorD-glucoseDyeEnsembleViologens

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Area of Science:

  • Analytical Chemistry
  • Supramolecular Chemistry
  • Biomedical Sensing

Background:

  • Accurate D-glucose monitoring is crucial for managing diabetes and other metabolic disorders.
  • Existing glucose sensing methods often face challenges with sensitivity, selectivity, or reversibility.
  • Fluorescent probes offer potential for sensitive and real-time detection.

Purpose of the Study:

  • To design and construct novel fluorescent ensembles for sensitive D-glucose detection.
  • To investigate the sensing performance of these ensembles, focusing on sensitivity and reversibility.
  • To establish a new paradigm for developing highly selective glucose probes.

Main Methods:

  • Fabrication of three fluorescent ensembles combining a fluorescent dye (NAHBDS) with boronic acid substituted viologens (BBVs) as quenchers/receptors.
  • Evaluation of D-glucose sensing capabilities using fluorescence spectroscopy in a pH 7.4 buffer solution.
  • Analysis of the sensing mechanism, including reversibility and linear response to low D-glucose concentrations.

Main Results:

  • The NAHBDS/o-BBV and NAHBDS/m-BBV ensembles demonstrated high sensitivity towards D-glucose.
  • A reversible 'on-off' fluorescence response was observed, indicating dynamic interaction with D-glucose.
  • The recovery of fluorescence intensity showed a good linear relationship with low D-glucose concentrations.

Conclusions:

  • The developed ensembles provide a sensitive and reversible platform for D-glucose detection.
  • The sensing mechanism operates via a dynamically reversible equilibrium process.
  • This research presents a novel strategy for the design of highly selective fluorescent probes for D-glucose.