Symbiotic seed germination and protocorm development of Aa achalensis Schltr., a terrestrial orchid endemic from Argentina
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Updated: May 10, 2026

Asymbiotic Germination and Leaf Explant-Based Regeneration of the Endangered Medicinal Orchid Hemipilia cucullata from Mature Seeds
Published on: September 19, 2025
Fracchia Sebastián1, Silvani Vanesa, Flachsland Eduardo
1Centro Regional de Investigaciones Científicas y Transferencia Tecnológica, CRILAR- CONICET, Entre Ríos y Mendoza, 5301, Anillaco, La Rioja, Argentina, sebafrac@yahoo.com.ar.
Abstract:
Aa achalensis is an endangered terrestrial orchid endemic from Argentina. In vitro symbiotic seed germination was evaluated for its propagation. Five different fungal strains were isolated from this species: two Rhizoctonia-like related to Thanatephorus cucumeris and three ascomicetaceous fungi belonging to Phialophora graminicola and one to an uncultured Pezizaceae. All five isolates promoted seed germination being one T. cucumeris strain the most effective. After 16 weeks of growth, 30% of A. achalensis protocorms developed until seedlings with two/four leaves in this treatment. These findings open an opportunity to the knowledge and preservation of this species.
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