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Engineering Three-dimensional Epithelial Tissues Embedded within Extracellular Matrix
08:49

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Published on: July 10, 2016

Region-specific epithelial cell dynamics during branching morphogenesis.

Jeff C Hsu1, Hyun Koo, Jill S Harunaga

  • 1Cell Biology Section, Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|June 20, 2013
PubMed
Summary
This summary is machine-generated.

Epithelial cell motility in developing salivary glands is highest at the basement membrane, driven by integrins and myosin II. Inner bud cell migration is regulated by E-cadherin, impacting tissue organization.

Keywords:
E-cadherinKikGRbasement membranecell migrationintegrinmyosin IIorgan morphogenesissalivary gland

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Tissue Engineering

Background:

  • Epithelial cells in developing organs exhibit significant motility during branching morphogenesis.
  • The precise movements and molecular mechanisms governing epithelial cell migration in these processes remain incompletely understood.

Purpose of the Study:

  • To characterize the motility of epithelial cells in developing salivary glands.
  • To identify molecular factors regulating cell migration in different regions of the developing gland.

Main Methods:

  • Generated transgenic mice expressing photo-convertible KikGR for cell tracking.
  • Utilized fluorescence microscopy to track individual cell movements in vivo.
  • Applied antibodies against α6+β1 integrins and blebbistatin (nonmuscle myosin II inhibitor), and inhibited E-cadherin to assess their roles in cell migration.

Main Results:

  • Outer bud epithelial cells adjacent to the basement membrane showed the highest motility and were pleomorphic.
  • Inner bud cells exhibited lower motility and were rounded, while duct cells had the lowest motility.
  • Motility of outer cells was dependent on α6+β1 integrins and nonmuscle myosin II, but not E-cadherin.
  • Inner bud cell migration was enhanced by inhibiting E-cadherin.

Conclusions:

  • Basement membrane association is crucial for high epithelial cell motility in developing salivary glands.
  • Integrins and myosin II mediate basement membrane-dependent cell motility, while E-cadherin restrains inner bud cell migration.
  • These findings highlight the distinct molecular regulation of cell motility based on location within the developing salivary gland, impacting tissue organization and morphology.