A simple and precise method for quantitative analysis of lumefantrine by planar chromatography

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis
• Chromatography

Background:

• Malaria, particularly P. falciparum, remains a significant global health challenge.
• Accurate quantification of antimalarial drugs like Lumefantrine is crucial for effective treatment and quality control.
• Existing analytical methods may lack the sensitivity, precision, or simplicity required for routine pharmaceutical analysis.

Purpose of the Study:

• To develop and validate a simple, precise, and sensitive High-Performance Thin-Layer Chromatographic (HPTLC) method.
• To enable accurate quantitative analysis of Lumefantrine, a primary drug for treating P. falciparum malaria.
• To establish a reliable method for routine quality control of Lumefantrine in pharmaceutical dosage forms.

Main Methods:

• Utilized Silica gel 60 F254 HPTLC precoated plates for quantitative analysis.
• Employed a solvent system of Methanol:Water (9.5 + 0.5, v/v).
• Performed densitometric scanning using a deuterium lamp at a detection wavelength of 266 nm.

Main Results:

• The developed HPTLC method demonstrated linearity for Lumefantrine concentration in the range of 1.25-12.50 μg/ml.
• Validation of the method confirmed its suitability according to ICH guidelines (Q2B).
• The method proved to be simple, sensitive, precise, and accurate.

Conclusions:

• The validated HPTLC method is suitable for the quantitative analysis of Lumefantrine.
• This method can be effectively applied to the routine quality control of Lumefantrine in commercial pharmaceutical products.
• The study provides a robust analytical tool for ensuring the quality and efficacy of Lumefantrine antimalarial treatments.