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Updated: May 10, 2026

Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section
Published on: June 20, 2025
Simple spectrophotometric method for estimation of disodium edetate in topical gel formulations
Sunil Kamboj1, Deepak Sharma, Anroop B Nair
1Department of Pharmaceutics, M. M. College of Pharmacy, M. M. University, Mullana, Ambala, Haryana, India.
Abstract:
A simple, sensitive, cost-effective and reproducible UV-spectrophotometric method has been developed and validated for the estimation of disodium edetate in topical gel formulations. Solution of disodium edetate reacts with ferric chloride to form complex in 0.1 N HCl giving λmax at 270 nm. Beer's law was obeyed in the concentration range of 5-50 μg/mL (r (2)= 0.9997). The limit of detection and limit of quantitation were found to be 1.190 and 3.608 μg/mL, respectively. The results show that the procedure is accurate, precise, and reproducible (relative standard deviation < 1%), while being simple and less time consuming. The study concluded that the UV-spectrophotometric method could be used for the quantification of disodium edetate in pure form as well as in pharmaceutical formulations.
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