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Application of UV spectrophotometric method for easy and rapid estimation of lafutidine in bulk and pharmaceutical
Kiran Jadhav1, Dinesh Dhamecha, Amol Tate
1Department of Pharmaceutical Analysis, Genba Sopanrao Moze College of Pharmacy, Wagholi, Pune, India.
Introduction:
The present research work discusses the development of a UV estimation method for lafutidine. Simple, accurate, cost efficient, and reproducible spectrophotometric method has been developed for the estimation of Lafutidine in bulk and pharmaceutical dosage form.
Materials And Methods:
The Stock solution was prepared in a mixture of water and methanol (1:1). Further dilutions were made in water.
Results:
The drug was determined at maximum wavelength (λmax) 279 nm. Beers law was obeyed in the concentration range of 10-50 μg/ml having line equation y = 0.0100x + 0.035 with correlation coefficient of 0.999. Results of the analysis were validated statistically and by recovery study.
Conclusion:
The result of analysis was validated as per ICH guidelines and this method can be used for the routine analysis of lafutidine formulation.
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