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Updated: May 10, 2026

Stromal Vascular Fraction-enriched Fat Grafting for the Treatment of Symptomatic End-neuromata

Stromal Vascular Fraction-enriched Fat Grafting for the Treatment of Symptomatic End-neuromata

Published on: November 23, 2017

Nanofat grafting: basic research and clinical applications.

Patrick Tonnard¹, Alexis Verpaele, Geert Peeters

¹Ghent and Brussels, Belgium From Plastic and Aesthetic Surgery, Coupure Centrum for Plastic Surgery Ghent; the Department of Plastic Surgery, University Hospital of Brussels; and the Department of Basic Medical Sciences, Ghent University.

Plastic and Reconstructive Surgery

|June 21, 2013

Summary

Nanofat grafting, using processed fat, effectively rejuvenates skin without viable fat cells, preserving stem cells for improved quality. This technique shows promising results for skin treatments.

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Area of Science:

Regenerative Medicine
Aesthetic Surgery
Tissue Engineering

Background:

Fat grafting indications are expanding, with microfat grafting utilizing thin cannulas.
This study introduces nanofat, processed fat injected via ultra-thin needles (up to 27 gauge).
Preliminary cellular analysis of nanofat is presented.

Observation:

Nanofat grafting was applied in 67 cases for rhytides, scars, and under-eye treatments.
Three fat samples (macrofat, microfat, nanofat) were analyzed for cellular content and viability.
Adipocyte viability was assessed, alongside quantification of stromal vascular fraction and CD34+ cells.

Findings:

No viable adipocytes were detected in nanofat; however, adipose-derived stem cells remained abundant.
Stem cells from all samples exhibited comparable proliferation and differentiation capabilities.
Clinical outcomes demonstrated significant improvements in skin quality six months post-procedure, with no adverse effects.

Implications:

Nanofat injections represent a novel approach in lipofilling and skin rejuvenation.
The technique shows potential for enhancing skin quality in clinical aesthetic applications.
Further research into stem cell behavior and long-term efficacy is warranted.