Related Experiment Video

Updated: May 10, 2026

Application of the Intelligent High-Throughput Antimicrobial Sensitivity Testing/Phage Screening System and Lar Index of Antimicrobial Resistance
09:59

Application of the Intelligent High-Throughput Antimicrobial Sensitivity Testing/Phage Screening System and Lar Index of Antimicrobial Resistance

Published on: July 21, 2023

Susceptibility breakpoint for enrofloxacin against swine Salmonella spp

Haihong Hao1, Huafang Pan, Ijaz Ahmad

  • 1National Reference Laboratory of Veterinary Drug Residues/MOA Key Laboratory of the Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China.

Journal of Clinical Microbiology
|June 21, 2013
PubMed

Insights

This study establishes the first enrofloxacin susceptibility breakpoint for swine Salmonella, setting a MIC of ≤ 0.25 μg/ml. This provides a vital criterion for antimicrobial susceptibility testing and clinical use in pigs.

Area of Science:

  • Veterinary Microbiology
  • Antimicrobial Resistance
  • Food Animal Health

Background:

  • Prudent antimicrobial use is essential in livestock production.
  • Salmonella spp. in swine pose a significant food safety and animal health challenge.
  • Enrofloxacin is a critical antimicrobial for treating bacterial infections in food animals.

Purpose of the Study:

  • To establish the first susceptibility breakpoint for enrofloxacin against swine Salmonella spp.
  • To provide a reliable criterion for enrofloxacin susceptibility testing in veterinary diagnostics.
  • To guide the clinical application of enrofloxacin in swine Salmonella infections.

Main Methods:

  • Determination of wild-type cutoff (COWT) values.
  • Calculation of pharmacokinetic-pharmacodynamic (PK-PD) cutoff (COPD) values.
  • MIC determination for enrofloxacin against swine Salmonella isolates.

Main Results:

  • The first susceptibility breakpoint for enrofloxacin against swine Salmonella spp. was developed.
  • The established breakpoint is a minimum inhibitory concentration (MIC) of ≤ 0.25 μg/ml.
  • This breakpoint is based on both COWT and COPD values.

Conclusions:

  • A validated susceptibility breakpoint for enrofloxacin in swine Salmonella is now available.
  • This breakpoint facilitates accurate susceptibility testing and informed clinical decisions.
  • This contributes to responsible antimicrobial stewardship in swine production.

More Related Videos

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli12:29

Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli

Published on: March 24, 2023

Related Concept Videos

Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Inhibitors of Bacterial DNA Synthesis01:28

Inhibitors of Bacterial DNA Synthesis

Bacterial pathogens depend on precise and efficient DNA replication to sustain infection. Two type II topoisomerases—DNA gyrase and topoisomerase IV—are critical to this process, as they resolve DNA supercoiling and unlink chromosomes during replication. Fluoroquinolones, synthetic derivatives of quinolones, exploit this mechanism by stabilizing the transient DNA–enzyme cleavage complex, preventing strand religation, and causing lethal double-strand breaks. These antibiotics are selectively...