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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: May 10, 2026

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
14:48

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

Unveiling the rat urinary proteome with three complementary proteomics approaches.

Fernando Sánchez-Juanes1, María Carmen Muñiz, César Raposo

  • 1Unidad de Investigación, Hospital Universitario de Salamanca, Salamanca, Spain.

Electrophoresis
|June 21, 2013
PubMed
Summary

Researchers analyzed the rat urinary proteome, identifying 366 unique proteins. This comprehensive database aids in studying kidney function and discovering disease biomarkers.

Keywords:
MALDI-TOFPF2DRat urineTwo-dimensional electrophoresisUrinary proteome

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Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

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Last Updated: May 10, 2026

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
14:48

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
07:38

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Published on: April 11, 2019

Area of Science:

  • Proteomics
  • Biomarker Discovery
  • Renal Physiology

Background:

  • Urine is an accessible biofluid for identifying disease markers.
  • Rats are common models for human diseases, but their urinary proteome is understudied.
  • A comprehensive understanding of the rat urinary proteome is crucial for translational research.

Purpose of the Study:

  • To comprehensively analyze the normal rat urinary proteome.
  • To establish the most extensive rat urinary protein database.
  • To evaluate the complementarity of different proteomics techniques.

Main Methods:

  • Utilized three complementary proteomics platforms: SDS-PAGE with LC-ESI-MS/MS, 2DE with MALDI-TOF-TOF, and 2D-LC-chromatofocusing with LC-ESI-Q-TOF.
  • Analyzed rat urine samples to identify and quantify proteins.
  • Compared protein identification across the different platforms.

Main Results:

  • Identified a total of 366 unique proteins in the rat urine.
  • Observed significant complementarity, with only 5.2% of proteins identified by all three methods.
  • Demonstrated that combined proteomics approaches yield non-redundant information.

Conclusions:

  • The study provides the most extensive database of rat urinary proteins to date.
  • Complementary proteomics techniques are essential for thorough proteome analysis.
  • This resource will advance research in renal physiology and biomarker discovery for renal and nonrenal diseases.