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Updated: May 10, 2026

Microfluidic Co-culture of Epithelial Cells and Bacteria for Investigating Soluble Signal-mediated Interactions
12:25

Microfluidic Co-culture of Epithelial Cells and Bacteria for Investigating Soluble Signal-mediated Interactions

Published on: April 20, 2010

Patterning bacterial communities on epithelial cells.

Mohammed Dwidar1, Brendan M Leung, Toshiyuki Yaguchi

  • 1School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea.

Plos One
|June 21, 2013
PubMed
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This summary is machine-generated.

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Aqueous two-phase systems (ATPS) enable precise bacterial patterning to study host-pathogen interactions. Invasive bacteria damage epithelial cells, while predators like Bdellovibriobacteriovorus HD 100 can selectively remove bacteria to protect host cells.

Area of Science:

  • Microbiology
  • Cell Biology
  • Biotechnology

Background:

  • Bacterial interactions with host cells are crucial for understanding infection and disease.
  • Current methods for studying localized bacterial effects are limited.
  • Micropatterning techniques offer new ways to investigate these interactions.

Purpose of the Study:

  • To develop and apply a novel method for localized bacterial culture and community formation on epithelial cells.
  • To compare the pathogenic effects of invasive and non-invasive *Escherichia coli* strains.
  • To demonstrate the selective removal of bacterial communities using a predatory bacterium.

Main Methods:

  • Utilized aqueous two-phase systems (ATPS) for precise micropatterning of bacteria on human epithelial cell sheets.

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Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
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Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

Published on: December 17, 2015

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Last Updated: May 10, 2026

Microfluidic Co-culture of Epithelial Cells and Bacteria for Investigating Soluble Signal-mediated Interactions
12:25

Microfluidic Co-culture of Epithelial Cells and Bacteria for Investigating Soluble Signal-mediated Interactions

Published on: April 20, 2010

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
12:16

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

Published on: December 17, 2015

  • Compared the effects of *E. coli* MG1655 and an invasive isogenic counterpart expressing invasin.
  • Assessed the localized impact of *E. coli*, *Shigella boydii*, and *Pseudomonas* sp. on epithelial cells.
  • Employed *Bdellovibriobacteriovorus* HD 100 for selective bacterial community removal.

    • Main Results:

    • Invasive *E. coli* caused significant epithelial cell damage and detachment, unlike non-invasive strains.
    • Localized effects of three different bacterial species on epithelial cells were successfully demonstrated.
    • *Bdellovibriobacteriovorus* HD 100 selectively removed bacterial communities.
    • Predation of *Pseudomonas* sp. was critical for maintaining underlying epithelial cell viability.

    Conclusions:

    • ATPS-based bacterial micropatterning is a powerful tool for studying bacteria-epithelial cell interactions.
    • This technique allows for the investigation of pathogen virulence and host responses in a controlled manner.
    • Bacterial predation offers a potential strategy for mitigating pathogen-induced host cell damage.