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DNA Base Pairing

Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,

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On the interaction between [Ru(NH3)6]3+ and the G-quadruplex forming thrombin binding aptamer sequence.

Aurore De Rache1, Thomas Doneux, Iva Kejnovská

  • 1Chimie Analytique et Chimie des Interfaces, Faculté des Sciences, Université Libre de Bruxelles, CP 255, Boulevard du Triomphe 2, B-1050 Bruxelles, Belgium.

Journal of Inorganic Biochemistry
|June 22, 2013
PubMed
Summary

The thrombin binding aptamer (TBA) interacts with hexaammineruthenium(III) cations, forming a stable complex on gold surfaces. This specific binding, with a 2:1 stoichiometry, is crucial for aptasensor development.

Keywords:
BiosensorsCircular dichroismG-quadruplexHexaamminerutheniumThrombin binding aptamerVoltammetry

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Area of Science:

  • Biophysical Chemistry
  • Nanotechnology
  • Analytical Chemistry

Background:

  • The thrombin binding aptamer (TBA) is a G-quadruplex DNA structure.
  • Hexaammineruthenium(III) ([Ru(NH3)6](3+)) is an electroactive cation used in biosensing.
  • Aptasensors rely on specific aptamer-analyte interactions for detection.

Purpose of the Study:

  • To investigate the interaction between TBA and [Ru(NH3)6](3+).
  • To characterize the binding stoichiometry and nature of the interaction.
  • To evaluate the potential of this interaction in aptasensor applications.

Main Methods:

  • Electrochemical methods (e.g., differential pulse voltammetry).
  • Circular dichroism (CD) spectroscopy.
  • Surface immobilization of TBA on gold electrodes.

Main Results:

  • A specific binding stoichiometry of 2 [Ru(NH3)6](3+) cations per TBA strand was determined.
  • The interaction is specific and not a general electrostatic charge compensation.
  • [Ru(NH3)6](3+) remains bound to immobilized TBA even in the absence of the cation in solution.

Conclusions:

  • The study reveals a specific interaction between TBA and hexaammineruthenium(III) cations.
  • This specific binding is suitable for aptasensor development, enabling stable immobilization of the redox marker.
  • The findings have implications for designing electrochemical biosensors with enhanced stability and sensitivity.