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Related Experiment Video

Updated: May 10, 2026

Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells

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Adjustable trapping position for single cells using voltage phase-controlled method.

Chun-Chih Wang¹, Kung-Chieh Lan, Ming-Kun Chen

¹Department of Electrical Engineering and Center for Micro/Nano Science and Technology, National Cheng Kung University, 1 University Road, Tainan 701, Taiwan.

Biosensors & Bioelectronics

|June 22, 2013

Summary

This summary is machine-generated.

This study introduces a novel biochip particle trap using voltage phase-controlled (VPC) method and negative dielectrophoresis (nDEP) for precise cell manipulation in physiological media. The technique allows adjustable 3D positioning of single particles and cells.

Keywords:

Adjustable trapping position Cell impedance measurement Cell manipulation Dielectrophoresis (DEP)Voltage phase-controlled method (VPC)

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Area of Science:

Biotechnology
Microfluidics
Biophysics

Background:

Micron-sized particle trapping is crucial for biochip systems.
Existing methods face limitations in high conductivity media and precise positioning.

Purpose of the Study:

To develop an advanced technique for adjustable particle/cell trapping in biochips.
To utilize voltage phase-controlled (VPC) method and negative dielectrophoresis (nDEP) in physiological media.

Main Methods:

Designed a planar, split micro-electrode structure for independent measurement and trapping.
Employed VPC and nDEP theory for particle manipulation.
Conducted numerical simulations and experimental verification.

Main Results:

Achieved adjustable trapping positions in vertical (0-26 μm) and horizontal (0-74 μm) directions.
Demonstrated precise single particle/cell capture, measurement, and release.
Maintained impedance error below 5% with the proposed electrode layout.

Conclusions:

The developed technique enables precise, adjustable 3D trapping of particles/cells in biochips.
VPC and nDEP are effective for manipulation in high conductivity media.
The system shows high precision and reliability for bio-analytical applications.