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Updated: May 10, 2026
Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip
Published on: July 7, 2012
A quantitative ratiometric sensor for time-resolved analysis of auxin dynamics
Sabrina Wend1, Cristina Dal Bosco, Michael M Kämpf
1Faculty of Biology, University of Freiburg, Freiburg, Germany.
Abstract:
Time-resolved quantitative analysis of auxin-mediated processes in plant cells is as of yet limited. By applying a synergistic mammalian and plant synthetic biology approach, we have developed a novel ratiometric luminescent biosensor with wide applicability in the study of auxin metabolism, transport, and signalling. The sensitivity and kinetic properties of our genetically encoded biosensor open new perspectives for the analysis of highly complex auxin dynamics in plant growth and development.
