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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: May 10, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Published on: June 28, 2017

A light sheet based high throughput 3D-imaging flow cytometer for phytoplankton analysis.

Jianglai Wu¹, Jianping Li, Robert K Y Chan

¹Department of Physics, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China.

|June 22, 2013

Summary

This summary is machine-generated.

A novel light sheet fluorescence imaging flow cytometer enables 3D phytoplankton analysis. This high-throughput instrument offers high spatial resolution for improved phytoplankton taxonomy.

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Last Updated: May 10, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Published on: June 28, 2017

Characterization of Aquatic Biofilms with Flow Cytometry

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Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

Area of Science:

Marine biology
Microscopy
Cytometry

Background:

Phytoplankton taxonomy relies on detailed morphological analysis.
Traditional methods can be time-consuming and lack 3D structural information.
Advancements in imaging and flow cytometry offer potential for improved analysis.

Purpose of the Study:

To develop and report a light sheet fluorescence imaging flow cytometer.
To enable 3D sectioning and high-resolution imaging of phytoplankton.
To assess the instrument's potential for phytoplankton taxonomy.

Main Methods:

Development of a light sheet fluorescence imaging flow cytometer.
Integration of flow cytometry principles with light sheet microscopy.
Quantification of sample volume flow rate at 0.5 μl/min.
3D imaging of internal chlorophyll-a structure in dinoflagellates.

Main Results:

The instrument achieves high cell counting throughput.
High spatial resolution comparable to light sheet microscopy is obtained.
Preliminary 3D morphological data of internal chlorophyll-a structure were acquired for two dinoflagellate species.

Conclusions:

The developed instrument shows promising potential for phytoplankton taxonomy.
3D morphological analysis can enhance species identification and classification.
The method offers a new approach for studying phytoplankton structure and diversity.