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Updated: May 10, 2026
Light Sheet Microscopy of Fast Cardiac Dynamics in Zebrafish Embryos
Published on: August 13, 2021
High frequency photoacoustic imaging for in vivo visualizing blood flow of zebrafish heart
Jinhyoung Park1, Thomas M Cummins, Michael Harrison
1NIH Resource on Medical Ultrasonic Transducer Technology, Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USA.
Abstract:
A technique on high frame rate(28fps), high frequency co-registered ultrasound and photoacoustic imaging for visualizing zebrafish heart blood flow was demonstrated. This approach was achieved with a 40MHz light weight(0.38g) ring-type transducer, serving as the ultrasound transmitter and receiver, to allow an optic fiber, coupled with a 532nm laser, to be inserted into the hole. From the wire target study, axial resolutions of 38µm and 42µm were obtained for ultrasound and photoacoustic imaging, respectively. Carbon nanotubes were utilized as contrast agents to increase the flow signal level by 20dB in phantom studies, and zebrafish heart blood flow was successfully observed.
