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Updated: May 10, 2026

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
Published on: September 16, 2022
Off-axis setup taking full advantage of incoherent illumination in coherence-controlled holographic microscope
Tomáš Slabý1, Pavel Kolman, Zbyněk Dostál
1Institute of Physical Engineering, Faculty of Mechanical Engineering, Brno University of Technology, Technická 2, 616 69 Brno, Czech Republic. tomslaby@gmail.com
Abstract:
Coherence-controlled holographic microscope (CCHM) combines off-axis holography and an achromatic grating interferometer allowing for the use of light sources of arbitrary degree of temporal and spatial coherence. This results in coherence gating and strong suppression of coherent noise and parasitic interferences enabling CCHM to reach high phase measurement accuracy and imaging quality. The achievable lateral resolution reaches performance of conventional widefield microscopes, which allows resolving up to twice smaller details when compared to typical off-axis setups. Imaging characteristics can be controlled arbitrarily by coherence between two extremes: fully coherent holography and confocal-like incoherent holography. The basic setup parameters are derived and described in detail and experimental validations of imaging characteristics are demonstrated.
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