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Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis

Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis

Published on: September 29, 2023

Advances in lectin microarray technology: optimized protocols for piezoelectric print conditions.

Kanoelani T Pilobello¹, Praveen Agrawal, Richard Rouse

¹Department of Chemistry, Biomedical Chemistry Institute, New York University, New York, USA.

Current Protocols in Chemical Biology

|June 22, 2013

Summary

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Lectin microarrays profile glycans for clinical biomarkers. A new non-contact piezoelectric printing method enhances lectin activity, improving glycan analysis for glycobiology research.

Area of Science:

Glycobiology
Biomarker Discovery
Analytical Chemistry

Background:

Lectin microarray technology enables glycan profiling of biological and clinical samples.
This technology has led to advancements in glycobiology and the identification of clinical biomarkers.
Current lectin microarrays utilize >90 plant lectins, recombinant lectins, and antibodies to profile various glycan types.

Purpose of the Study:

To present an updated protocol for lectin microarray production using a non-contact piezoelectric printer.
To demonstrate how this new method enhances lectin activity on the array compared to previous contact printing methods.
To discuss the optimization of printing, sample hybridization, and analysis conditions for improved glycan profiling.

Main Methods:

Keywords:

Nano‐Plotter carbohydrate analysis glycomics lectin microarray piezoelectric

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Last Updated: May 10, 2026

Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis

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Utilized a non-contact, piezoelectric printer for lectin microarray production.

Optimized print conditions and sample hybridization parameters.

Developed and discussed methods for analyzing the resulting glycan profiles.

Main Results:

The piezoelectric printing method resulted in increased lectin activity on the microarray.
The updated protocol facilitates more effective glycan profiling.
The study provides a refined approach for lectin microarray fabrication and application.

Conclusions:

Non-contact piezoelectric printing offers an improved method for lectin microarray production.
Enhanced lectin activity on the array leads to more robust glycan profiling.
This optimized technology advances the application of lectin microarrays in glycobiology and clinical biomarker discovery.