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Updated: May 10, 2026

Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons
Published on: January 18, 2019
[Desensitization of glycine mediated currents in frog spinal cord neurons]
Abstract:
Whole-cell patch clamp recordings from isolated frog spinal cord neurons were made to study the desensitization of glycine-mediated currents. The decay phase of current induced by application of glycine (1 mM) could be either monoexponential or biexponential. Monoexponential decays had either tau1 = 1693 +/- 135 ms (n = 8) or tau2 = 364 +/- 42 ms (n = 9) time constants, while biexponential decays had both tau1 and tau2. Currents with fast monoexponential decay (tau2) remained reproducible through the experiment. Currents with slow monoexponential (tau1) and biexponential decay gradually became faster while whole-cell patch clamp recordings were being taken. These results suggest that, at least, two different glycine receptor subtypes are present in frog spinal cord neurons.
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