Related Experiment Video
Updated: May 10, 2026

Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System
Published on: January 30, 2019
Determination of aminothiols by liquid chromatography with amperometric detection at a silver electrode: application
Ahmad Sarakbi1, Zeynep Aydogmus, Angela Dago
1Université Libre de Bruxelles, Faculty of Pharmacy, Brussels, Belgium.
Abstract:
Liquid chromatography coupled to a silver electrode based flow-through amperometric detector (LC-EC-Ag) was developed for the determination of aminothiols in white wines. The C18 reversed phase LC system operated in the isocratic mode at 0.7 mL min(-1) and used an acidic mobile phase composed of formic acid, EDTA, sodium nitrate, sodium hydroxide, and methanol 1% (v/v) at pH 4.5. The working electrode operated at 0.08 V vs Ag/AgCl, 3M KCl and its manual cleaning was realized once a month by smoothing on a polishing cloth. The analyzed aminothiols were resolved and eluted within 4 min, and all standard curves were linear in the range 2×10(-7)-2×10(-5) M. The analyzed wine samples needed no preparation other than dilution with the mobile phase. The concentration of cysteine (CYS), homocysteine (HCYS), glutathione (GSH) and N-acetylcysteine (NAC) in bottled white wines, determined by the method of standard addition, was found to be in the low μM range (0.2-2 mg L(-1)) depending on the wine type and its age.
Related Concept Videos
Precipitation Titration: Endpoint Detection Methods
In the Volhard method, a standard excess of AgNO3 is first added to the...
Effects of EDTA on End-Point Detection Methods
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...
High-Performance Liquid Chromatography: Types of Detectors
EDTA: Indirect and Alkalimetric Titration
Amperometry: Overview
Precipitation Titration: Overview
A precipitation titration curve demonstrates the change in concentration of the titrant or analyte upon adding the...

