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Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Related Experiment Video

Updated: May 2, 2026

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Published on: July 31, 2011

Quantitation of endogenous peptides using mass spectrometry based methods.

Elena V Romanova¹, Sarah E Dowd, Jonathan V Sweedler

¹Department of Chemistry and the Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Current Opinion in Chemical Biology

|June 25, 2013

Summary

Quantitative peptidomics uses mass spectrometry to discover peptides for cell communication, aiding biomarker and drug development. Advances in quantitative methods and data analysis accelerate peptide research and functional characterization.

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Last Updated: May 2, 2026

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

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Area of Science:

Biochemistry
Analytical Chemistry
Molecular Biology

Background:

Peptidomics, a mass spectrometry sub-discipline, analyzes endogenous peptides in cell communication.
Significant advancements have been made in bioanalytical technologies for peptide discovery.
Robust quantitative mass spectrometry and data mining are key to recent progress.

Purpose of the Study:

To outline current quantitative peptidomics approaches.
To discuss technical challenges driving innovation in the field.
To review recent literature on endogenous peptide functional characterization via quantitative mass spectrometry.

Main Methods:

Quantitative mass spectrometry
Data mining algorithms
Peptide discovery and validation techniques

Main Results:

Accelerated discovery and validation of biomarkers.
Identification of functionally important posttranslational modifications.
Uncovering of novel molecular interactions relevant to drug development.

Conclusions:

Quantitative peptidomics is a rapidly advancing field crucial for understanding cell communication.
Technological innovations in mass spectrometry and data analysis are key drivers of progress.
This field holds significant promise for advancing drug development and biomarker discovery.