Dynamic structural changes in microbial membranes in response to high hydrostatic pressure analyzed using

Related Experiment Video

Updated: May 10, 2026

High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
04:37

High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States

Published on: June 29, 2021

Fumiyoshi Abe1

  • 1Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University, Sagamihara, Japan; Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan.

Biophysical Chemistry
|June 25, 2013
PubMed

Keywords:
1,6-diphenyl-1,3,5-hexatriene1-[4-(trimethylamino)pheny]-6-phenyl-1,3,5-hexatrieneD(w)DHADOPCDPHDPPCDSPCDeep-sea piezophileEPAHP-TRFAMHigh-pressure time-resolved fluorescence anisotropy measurement (HP-TRFAM)Membrane fluidityPOPCPUFASSAPCSDPCSOPCTCSPCTMA-DPHdioleyl-phosphatidylcholinedipalmitoyl-phosphatidylcholinedistearoyl-phosphatidylcholinedocosahexaenoic acid (C22:6)eicosapentaenoic acid (C20:5)fluorescence anisotropyhigh-pressure time-resolved fluorescence anisotropy measurementorder parameterpalmitoyl-oleoyl-phosphatidylcholinepolyunsaturated fatty acidsrrotational diffusion coefficientstearoyl-arachidonoyl-phosphatidylcholinestearoyl-docosahexanoylphosphatidylcholinestearoyl-oleyl-phosphatidylcholinetime-correlated single-photon counting

More Related Videos

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions10:02

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

Published on: May 27, 2021

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules10:23

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

Published on: April 25, 2025

Related Concept Videos

Deep Sea Microbial Ecology01:18

Deep Sea Microbial Ecology

The deep ocean and its underlying sediments represent vast, largely unexplored microbial habitats that extend far beyond the sunlit photic zone. The photic (euphotic) zone typically spans the upper ~100–200 meters of pelagic waters in the open ocean, but its depth varies geographically and seasonally, where sufficient light supports photosynthetic life. Below this lies the deep sea, spanning roughly 1000–6000 meters (bathypelagic to abyssal zones), with deeper hadal trenches extending beyond...
Biosynthesis of Lipids01:29

Biosynthesis of Lipids

Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis pathway, which...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...