Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

M-Cdk Drives Transition Into Mitosis

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

M-Cdk Drives Transition Into Mitosis

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a rapamycin-insensitive companion...

Negative Regulator Molecules

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.

Molecular Factors Affecting Cell Division

Molecular Factors Affecting Cell Division

Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...

Positive Regulator Molecules

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Red ginseng-derived nanovesicles to modulate osteoblast and osteoclastogenesis for osteoporosis therapy.

Asian journal of pharmaceutical sciences·2026

Same author

Silencing of β-TrCP2 Suppresses Growth and G<sub>1</sub>/S Cell Cycle Progression in Non-small Cell Lung Carcinoma Cells.

Anticancer research·2026

Same author

Therapeutic potential of panax ginseng-derived nanovesicles in osteoporosis through enhanced osteoblast.

Journal of ginseng research·2026

Same author

Symptom Response Dynamics for Personalised Therapy with Subcutaneous Infliximab in Crohn's Disease: Insights from the Randomised, Placebo-Controlled, Phase 3 LIBERTY-CD Trial.

Advances in therapy·2026

Same author

TACE reprograms RANKL-mediated differentiation of macrophages by activating the non-canonical pathway of IRF3.

bioRxiv : the preprint server for biology·2026

Same author

CTRP1 regulates skeletal muscle differentiation through quality control of mitochondrial dynamics and function.

Molecular therapy : the journal of the American Society of Gene Therapy·2026

Same journal

Epigallocatechin gallate suppresses adipogenesis by targeting tetranectin and inhibiting its endocytosis via modulation of ERK and AKT signaling pathways.

BMB reports·2026

Same journal

Immunoproteasome inhibition protects the retina from Alzheimer's-associated degeneration.

BMB reports·2026

Same journal

Urolithin A improves mitochondrial dysfunction induced by oxidative stress in human dermal papilla cells.

BMB reports·2026

Same journal

Whole genome-based polygenic risk score and structural variation analysis in Korean patients with inflammatory bowel disease.

BMB reports·2026

Same journal

EK-16005, a 2-anilinopyrimidine derivative, inhibits breast cancer progression by regulating myeloid-derived suppressor cells.

BMB reports·2026

Same journal

Immune phenotype-based stratification of colorectal cancer reveals subtype-specific immunotherapeutic opportunities: insights from a Korean patient cohort.

BMB reports·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 10, 2026

Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors

Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors

Published on: July 17, 2020

PP2A function toward mitotic kinases and substrates during the cell cycle.

Ae Lee Jeong¹, Young Yang

¹Center for Women's Disease, Department of Biological Science, Sookmyung Women's University, Seoul 140-742, Korea.

|June 25, 2013

Summary

This summary is machine-generated.

Precise regulation of kinases and phosphatases, including cyclin-dependent kinase 1 (CDK1):cyclin B, is vital for cell cycle progression. This study explores how these enzymes control cell division timing and localization.

More Related Videos

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

Related Experiment Videos

Last Updated: May 10, 2026

Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors

Assessing Cellular Target Engagement by SHP2 (PTPN11) Phosphatase Inhibitors

Published on: July 17, 2020

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Cellular homeostasis requires precise regulation of protein phosphorylation by kinases and phosphatases.
The cyclin-dependent kinase 1 (CDK1) and cyclin B complex (CDK1:cyclin B) is a key regulator of cell cycle progression.
CDK1:cyclin B activation involves auto-amplification loops, including activation of Cdc25 phosphatase and inhibition of Wee1 kinase.

Purpose of the Study:

To elucidate the role of phosphatase regulation in mitotic kinase activity and cell cycle control.
To understand the interplay between kinases and phosphatases in governing cell division.
To provide new insights into ordered cell cycle progression.

Main Methods:

Review of recent biological evidence on kinase and phosphatase regulation during mitosis.
Analysis of auto-amplification loops involving CDK1:cyclin B, Cdc25, and Wee1.
Examination of phosphatase counteracting activity during mitosis.

Main Results:

CDK1:cyclin B activation is accompanied by the inhibition of counteracting phosphatase activity.
Phosphatase regulation is crucial for the ordered progression of the cell cycle.
Mutual control between kinases and phosphatases dictates cell division timing and localization.

Conclusions:

The coordinated regulation of kinases and phosphatases is essential for maintaining cellular homeostasis and ensuring accurate cell division.
Understanding the feedback mechanisms governing kinase and phosphatase activity offers new perspectives on cell cycle regulation.
Further investigation into the localization and timing control by these enzyme systems is warranted.