Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transport Number01:31

Transport Number

The transport number is the fraction of the total current carried by an ion in an electrolyte solution. It is defined as the ratio of the current carried by a specific ion to the total current flowing through the solution. The transport number, t, is central to understanding ionic mobility, which describes how fast an ion moves under the influence of an electric field. This link connects the physical behavior of ions in solution to the chemical processes that occur during electrochemical...
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thermodynamics of imidazole-ligand binding to Ni-nitrilotriacetate in solution and covalently attached to agarose beads: imidazole, his-6 (his-tag) peptide and a new bis-imidazolo-dithiane.

Protein expression and purification·2013
Same author

Human GLTP: Three distinct functions for the three tryptophans in a novel peripheral amphitropic fold.

Biophysical journal·2010
Same author

DNA bending by charged peptides: electrophoretic and spectroscopic analyses.

Biochemistry·2007
Same author

The binding of 1,8 ANS congeners to I-FABP and comparison of some hypotheses about ANS' spectral sensitivity to environment.

Biochimica et biophysica acta·2005
Same author

Measurement of intermolecular distances for the natural agonist Peptide docked at the cholecystokinin receptor expressed in situ using fluorescence resonance energy transfer.

Molecular pharmacology·2004
Same author

Environment and mobility of a series of fluorescent reporters at the amino terminus of structurally related peptide agonists and antagonists bound to the cholecystokinin receptor.

The Journal of biological chemistry·2002
Same journal

Synthesis of covalent organic frameworks and plasmon-assisted exfoliation for enhanced solar hydrogen production.

Journal of colloid and interface science·2026
Same journal

Efficient hydrogen production and anti-coking via reforming of waste plastics by oxygen vacancy promoted plasma-catalysis.

Journal of colloid and interface science·2026
Same journal

Lanthanum-modulated hollow CuO nanofibers enable selective CO<sub>2</sub> electroreduction to multicarbon products at high current densities.

Journal of colloid and interface science·2026
Same journal

Tris(vinyl dimethylsilyl) phosphate: Enhancing interface stability in high-voltage Li-ion batteries at elevated temperatures.

Journal of colloid and interface science·2026
Same journal

Electron-donor modulated built-in electric fields in Ni<sub>2</sub>P/MoS<sub>2</sub> Heterostructures for accelerated sodium storage kinetics.

Journal of colloid and interface science·2026
Same journal

Porous flexible structure mediated synergistic boost of built-in electric field and photothermal effect for enhanced photocatalysis.

Journal of colloid and interface science·2026
See all related articles

Related Experiment Video

Updated: May 10, 2026

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence (TIRF) Microscopy
08:55

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence (TIRF) Microscopy

Published on: February 17, 2023

Transverse ionic mobility measured in a dynamic light scattering device.

William R Kirk1, William S Wessels

  • 1Mayo Clinic College of Medicine, Rochester, MN 55905, USA. wrkqdeformd@gmail.com

Journal of Colloid and Interface Science
|June 25, 2013
PubMed
Summary
This summary is machine-generated.

Researchers explored transverse ionic mobility using circularly polarized light with a dynamic light scattering device. This method reveals particle

Keywords:
Circular polarizationIonic mobilityNanoparticle electrophoretic mobility

More Related Videos

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
09:16

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy
08:17

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

Published on: August 16, 2021

Related Experiment Videos

Last Updated: May 10, 2026

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence (TIRF) Microscopy
08:55

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence (TIRF) Microscopy

Published on: February 17, 2023

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
09:16

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy
08:17

Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

Published on: August 16, 2021

Area of Science:

  • Colloid and Surface Science
  • Physical Chemistry
  • Nanotechnology

Background:

  • Dynamic Light Scattering (DLS) is a common technique for measuring particle size and zeta potential.
  • Standard DLS typically uses linearly polarized light, limiting the information obtainable about particle surface dynamics.
  • Chirality in particles or solvents can influence interactions with polarized light, but this is not fully exploited in standard DLS.

Purpose of the Study:

  • To investigate novel phenomena related to transverse ionic mobility in colloidal systems.
  • To explore the use of circularly polarized light (right-handed vs. left-handed) in DLS for enhanced surface characterization.
  • To understand the role of particle/solvent chirality and surface impedance in ionic mobility measurements.

Main Methods:

  • Modification of a commercial Malvern DLS 'Zetasizer' with a quarter-wave plate to generate circularly polarized light (RH and LH).
  • Utilizing the ionic mobility (zeta-potential) mode to measure particle movement under an applied static electric field.
  • Analyzing the scattered light phase shifts influenced by RH vs. LH polarized light interactions with particle surfaces.

Main Results:

  • Observed phenomena attributed to transverse ionic mobility, indicating particle movement perpendicular to the applied electric field.
  • Identified contributions from parity-biased mechanical flexing of charged moieties on chiral or achiral particles with chiral co-solvents.
  • Detected electro-optic effects from chiral co-solvents interacting with charged colloid surfaces in high-frequency electric fields.

Conclusions:

  • Circularly polarized light in DLS can probe transverse ionic mobility and internal particle dynamics ('floppiness').
  • Surface impedance phenomena, including mechanical flexing and electro-optic effects, significantly influence particle mobility in chiral environments.
  • This technique offers new insights into the complex interactions at the interface of colloidal particles and their surrounding medium.