Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
Cell Migration01:19

Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prediction of Mesenchymal Stromal Cell Immune Suppression Using Live Imaging in a Three-Dimensional Microfluidic Device.

ACS biomaterials science & engineering·2026
Same author

A microgel bone marrow model of mesenchymal stromal cell paracrine signaling supporting hematopoietic stem cell retention.

Acta biomaterialia·2026
Same author

Enabling volumetric printing of low viscosity hyaluronic acid-based resins through fast crosslinking reactions.

Biofabrication·2026
Same author

Engineering Corynebacterium glutamicum as a multifunctional biofactory for living therapeutic materials and controlled ectoine delivery.

Biomaterials advances·2026
Same author

Bone marrow-derived mesenchymal stromal cells yield greater pain relief and tissue protection than umbilical cord tissue-derived cells in a surgically induced instability model of osteoarthritis.

Osteoarthritis and cartilage·2026
Same author

Professor Xingdong Zhang Special Issue.

Tissue engineering. Part A·2026

Related Experiment Video

Updated: May 10, 2026

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
07:50

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification

Published on: June 2, 2020

Dynamic cell-adhesive microenvironments and their effect on myogenic differentiation.

Simone Weis1, Ted T Lee, Aránzazu del Campo

  • 1Max-Planck-Institut für Polymerforschung, Ackermannweg 10, 55128 Mainz, Germany.

Acta Biomaterialia
|June 25, 2013
PubMed
Summary

Controlling the density and timing of RGD peptide presentation on biomaterials significantly impacts cell adhesion, proliferation, and differentiation. Early RGD exposure within 6 hours maximizes C2C12 myoblast growth and myogenic differentiation.

Keywords:
BiomaterialCell adhesionFibronectinIntegrinRGD

More Related Videos

Fabrication and Use of MicroEnvironment microArrays (MEArrays)
11:57

Fabrication and Use of MicroEnvironment microArrays (MEArrays)

Published on: October 11, 2012

Sandwich-like Microenvironments to Harness Cell/Material Interactions
06:50

Sandwich-like Microenvironments to Harness Cell/Material Interactions

Published on: August 4, 2015

Related Experiment Videos

Last Updated: May 10, 2026

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
07:50

Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification

Published on: June 2, 2020

Fabrication and Use of MicroEnvironment microArrays (MEArrays)
11:57

Fabrication and Use of MicroEnvironment microArrays (MEArrays)

Published on: October 11, 2012

Sandwich-like Microenvironments to Harness Cell/Material Interactions
06:50

Sandwich-like Microenvironments to Harness Cell/Material Interactions

Published on: August 4, 2015

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Tissue Engineering

Background:

  • Integrin-mediated cell adhesion is crucial for cell behavior on biomaterial surfaces.
  • Understanding ligand presentation is key to controlling cell responses.

Purpose of the Study:

  • To investigate how RGD ligand density and presentation timing affect C2C12 myoblast adhesion, proliferation, and differentiation.
  • To explore temporal control over cell-biomaterial interactions.

Main Methods:

  • Utilized photoactivatable RGD peptides on PEGylated self-assembled monolayers.
  • Functionalized surfaces with RGD and caged RGD ligands.
  • Irradiated cultures at various time points (1-48h) to control RGD surface concentration.
  • Seeded with C2C12 myoblasts and analyzed attachment, spreading, proliferation, and differentiation.

Main Results:

  • Cell attachment, spreading, and myogenic differentiation were highly dependent on RGD surface density.
  • Proliferation and myogenesis peaked when RGD exposure occurred within 6 hours post-seeding.
  • Demonstrated temporal regulation of cell responses by ligand presentation timing.

Conclusions:

  • The density and temporal presentation of RGD ligands critically influence C2C12 myoblast behavior.
  • Early ligand presentation (≤6h) optimizes proliferation and myogenic differentiation.
  • Provides fundamental insights into temporal integrin-mediated cell responses for biomaterial design.