Related Experiment Video
Updated: May 10, 2026

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
Published on: May 16, 2011
Adhesive ability means inhibition activities for lactobacillus against pathogens and S-layer protein plays an
Wenming Zhang1, Haifeng Wang, Jianxin Liu
1College of Animal Science, MOE Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou 310029, PR China.
Abstract:
Eighty-five strains of lactobacillus were isolated from the pig intestine and identified by sequencing analysis based on 16S rRNA gene, from which five lactobacillus strains with high adhesive ability were selected. The inhibition ability of the five lactobacillus strains with or without S-layer proteins against adherence of Escherichia coli K88 and Salmonella enteritidis 50335 to Caco-2 was evaluated in vitro with Lactobacillus rhamnosus GG strain (LGG) as a positive control. In addition, tolerance of lactobacilli to heat, acid, bile, Zn(2+) and Cu(2+) were assessed. All five selected strains, Lactobacillus salivarius ZJ614 (JN981856), Lactobacillus reuteri ZJ616 (JN981858), L. reuteri ZJ617 (JN981859), L. reuteri ZJ621 (JN981863) and L. reuteri ZJ623 (JN981865), showed inhibition against the two pathogens, E. coli K88 and S. enteritidis 50335. L. reuteri ZJ621 showed higher inhibition ability than the others to S. enteritidis 50335 (P < 0.05). Sodium dodecyl sulfate-Polyacrylamide gel electrophoresis (SDS-PAGE) analysis indicated that all five strains had abundant bands with molecular weight ranging from 34 to 130 KDa as well as had a common band of approximately 42 KDa. After treatment with 5 M LiCl to remove S-layer protein, the inhibition activities of the lactobacilli against pathogens decreased significantly (P < 0.05). The results showed that higher adhesive ability means higher inhibition activity for lactobacillus against pathogen, in which S-layer proteins plays an important role.
Related Concept Videos
Outer Layers of the Cell Envelope
Laminins are the Adhesive Proteins of Basal Lamina
In humans, the five forms of alpha chains are LAMA 1, LAMA 2, LAMA 3, LAMA 4, and LAMA 5. The four forms of beta chains are LAMB 1, LAMB 2, LAMB 3, and LAMB 4. The three forms of gamma...
Adherens Junctions
Adherens Junctions are Dynamic
The endothelial cells...
Formation of Lipopolysaccharides
Cell Adhesion in Plants
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose, and...
Adhesion
Capillary action is a result of water’s adhesive tendencies. When a narrow glass...

