Related Experiment Video
Updated: May 10, 2026

Test Samples for Optimizing STORM Super-Resolution Microscopy
Published on: September 6, 2013
How good are my data and what is the resolution?
Philip R Evans1, Garib N Murshudov
1MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, England. pre@mrc-lmb.cam.ac.uk
Abstract:
Following integration of the observed diffraction spots, the process of `data reduction' initially aims to determine the point-group symmetry of the data and the likely space group. This can be performed with the program POINTLESS. The scaling program then puts all the measurements on a common scale, averages measurements of symmetry-related reflections (using the symmetry determined previously) and produces many statistics that provide the first important measures of data quality. A new scaling program, AIMLESS, implements scaling models similar to those in SCALA but adds some additional analyses. From the analyses, a number of decisions can be made about the quality of the data and whether some measurements should be discarded. The effective `resolution' of a data set is a difficult and possibly contentious question (particularly with referees of papers) and this is discussed in the light of tests comparing the data-processing statistics with trials of refinement against observed and simulated data, and automated model-building and comparison of maps calculated with different resolution limits. These trials show that adding weak high-resolution data beyond the commonly used limits may make some improvement and does no harm.
Related Concept Videos
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Chromatographic Resolution
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...
GIS Software, Hardware, and Sources of GIS Data
Super-resolution Fluorescence Microscopy
NMR Spectrometers: Resolution and Error Correction
Selected Data About Geographic Locations
