Identification of fungi species in the onychomycosis of institutionalized elderly
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Cidia Vasconcellos1, Carolina Queiroz Moreira Pereira, Marta Cristina Souza
1University of São Paulo, Medical School, São Paulo, Brazil. cidiav@ig.com.br
Background:
Superficial fungal infections are caused by dermatophytes, yeasts or filamentous fungi. They are correlated to the etiologic agent, the level of integrity of the host immune response, the site of the lesion and also the injured tissue.
Objective:
The purpose of this study is to isolate and to identify onychomycosis agents in institutionalized elderly (60 years old +).
Methods:
The identification of the fungi relied upon the combined results of mycological examination, culture isolation and micro cultures observation under light microscopy from nail and interdigital scales, which were collected from 35 elderly with a clinical suspicion of onychomycosis and a control group (9 elderly with healthy interdigital space and nails). Both groups were institutionalized in two nursing homes in Sao Bernardo do Campo, SP, Brazil.
Results:
The nail scrapings showed 51.40% positivity. Of these, dermatophytes were found in 44.40% isolates, 27.78% identified as Trichophyton rubrum and 5.56% each as Trichophyton tonsurans, Trichophyton mentagrophytes and Microsporum gypseum. The second more conspicuous group showed 38.89% yeasts: 16.67% Candida guilliermondii, 11.11% Candida parapsilosis, 5.56% Candida glabrata, and 5.56% Trichosporon asahii. A third group displayed 16.70% filamentous fungi, like Fusarium sp, Aspergillus sp and Neoscytalidium sp (5.56% each). The interdigital scrapings presented a positivity rate of 14.29%. The agents were coincident with the fungi that caused the onychomycosis. In the control group, Candida guilliermondii was found at interdigital space in one person.
Conclusion:
Employing a combination of those identification methods, we found no difference between the etiology of the institutionalized elderly onychomycosis from that reported in the literature for the general population.
Insights
This study identified common fungal agents causing onychomycosis in elderly individuals residing in nursing homes. The findings indicate that the prevalent fungi are similar to those found in the general population, suggesting no unique etiological differences in this demographic.
Area of Science:
- Medical Mycology
- Dermatology
- Gerontology
Background:
- Superficial fungal infections, including onychomycosis, are caused by dermatophytes, yeasts, or filamentous fungi.
- Infection severity depends on the causative agent, host immunity, lesion site, and tissue damage.
Purpose of the Study:
- To isolate and identify the causative agents of onychomycosis in institutionalized elderly individuals (aged 60 and above).
Main Methods:
- Collected nail and interdigital scales from 35 elderly individuals with suspected onychomycosis and 9 controls.
- Utilized mycological examination, culture isolation, and microscopy for fungal identification.
- Study conducted in two nursing homes in Sao Bernardo do Campo, SP, Brazil.
Main Results:
- Onychomycosis was detected in 51.40% of nail scrapings.
- Dermatophytes (44.40%) were the most common, including Trichophyton rubrum (27.78%).
- Yeasts (38.89%) and filamentous fungi (16.70%) were also identified, with agents matching interdigital infections.
Conclusions:
- Combined identification methods revealed no significant difference in onychomycosis etiology between institutionalized elderly and the general population.
- The study successfully identified common fungal pathogens responsible for onychomycosis in this specific elderly cohort.
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