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Updated: May 10, 2026

Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS
Published on: August 23, 2024
Multiplexed MS/MS for improved data-independent acquisition
Jarrett D Egertson1, Andreas Kuehn, Gennifer E Merrihew
1Department of Genome Sciences, University of Washington School of Medicine, Seattle, Washington, USA.
Abstract:
In mass spectrometry-based proteomics, data-independent acquisition (DIA) strategies can acquire a single data set useful for both identification and quantification of detectable peptides in a complex mixture. However, DIA data are noisy owing to a typical five- to tenfold reduction in precursor selectivity compared to data obtained with data-dependent acquisition or selected reaction monitoring. We demonstrate a multiplexing strategy, MSX, for DIA analysis that increases precursor selectivity fivefold.
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