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Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...

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Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS
07:12

Tracing de novo Lipids using Stable Isotope Labeling LC-TIMS-TOF MS/MS

Published on: August 23, 2024

Multiplexed MS/MS for improved data-independent acquisition.

Jarrett D Egertson1, Andreas Kuehn, Gennifer E Merrihew

  • 1Department of Genome Sciences, University of Washington School of Medicine, Seattle, Washington, USA.

Nature Methods
|June 25, 2013
PubMed
Summary
This summary is machine-generated.

Data-independent acquisition (DIA) in mass spectrometry produces noisy data. A new multiplexing strategy, MSX, enhances precursor selectivity fivefold for improved proteomics analysis.

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Published on: March 14, 2013

Area of Science:

  • Proteomics
  • Mass Spectrometry
  • Analytical Chemistry

Background:

  • Data-independent acquisition (DIA) is a mass spectrometry strategy for peptide identification and quantification.
  • DIA data can be noisy due to reduced precursor selectivity compared to other methods.
  • Improving precursor selectivity is crucial for enhancing DIA data quality.

Purpose of the Study:

  • To address the noise issue in DIA data.
  • To enhance precursor selectivity in DIA analysis.
  • To introduce a novel multiplexing strategy for DIA.

Main Methods:

  • Developed and implemented a multiplexing strategy termed MSX.
  • Applied MSX to DIA-based mass spectrometry.
  • Evaluated the impact of MSX on precursor selectivity.

Main Results:

  • The MSX strategy significantly increases precursor selectivity.
  • Achieved a fivefold increase in precursor selectivity using MSX.
  • Demonstrated the effectiveness of MSX in improving DIA data quality.

Conclusions:

  • MSX is an effective strategy for enhancing precursor selectivity in DIA proteomics.
  • This method offers a significant improvement over standard DIA acquisition.
  • MSX has the potential to improve peptide identification and quantification in complex mixtures.