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Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.

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Updated: May 10, 2026

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange

Published on: April 27, 2017

ESCAPE: database for integrating high-content published data collected from human and mouse embryonic stem cells.

Huilei Xu¹, Caroline Baroukh, Ruth Dannenfelser

¹Department of Pharmacology and Systems Therapeutics, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, Box 1215, New York, NY 10029, USA.

Database : the Journal of Biological Databases and Curation

|June 25, 2013

Summary

We developed the Embryonic Stem Cell Atlas from Pluripotency Evidence (ESCAPE) database to integrate diverse high-throughput data for mouse and human embryonic stem cells (m/hESCs). ESCAPE offers tools to explore molecular circuitry and regulatory interactions for stem cell systems biology research.

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Last Updated: May 10, 2026

Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange

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Published on: August 20, 2017

Area of Science:

Stem cell biology
Systems biology
Bioinformatics

Background:

Numerous high-content studies profile mouse and human embryonic stem cells (m/hESCs) using transcriptomics and proteomics.
Integrating these diverse datasets for a global molecular view remains a challenge.

Purpose of the Study:

To create a centralized, integrated database for m/hESC data.
To provide tools for exploring molecular circuitry and regulatory interactions.

Main Methods:

Integrated data from chromatin immunoprecipitation followed by deep sequencing, RNAi screens, gene expression, proteomics, and phosphoproteomics.
Developed a web-based interface with search, visualization, and network-building tools.
Implemented predictive tools for combinatorial knockdown effects.

Main Results:

The Embryonic Stem Cell Atlas from Pluripotency Evidence (ESCAPE) database is now available.
The database facilitates the identification of known and novel regulatory interactions across multiple layers.
Interactive tools allow for subnetwork construction and combinatorial knockdown effect prediction.

Conclusions:

ESCAPE serves as a comprehensive resource for the stem cell systems biology community.
The database enables deeper insights into the molecular mechanisms governing pluripotency.
Facilitates hypothesis generation and experimental design in stem cell research.