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Updated: May 10, 2026

Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry
Published on: September 3, 2010
Online spectral library for GC-atmospheric pressure chemical ionization-ToF MS
Tiziana Pacchiarotta1, Rico J E Derks, Elena Hurtado-Fernandez
1Centre for Proteomics & Metabolomics, Leiden University Medical Centre, Leiden, The Netherlands. t.pacchiarotta@lumc.nl
Background:
Invented more than three decades ago by Horning, GC-MS under atmospheric pressure chemical ionization (GC-APCI-MS) has only recently emerged from years of obscurity. However, the general acceptance of GC-APCI-MS is certainly constrained by the lack of spectral libraries, which make the traditional GC-MS approaches so powerful.
Results:
Here we present a concept of a GC-APCI-QqToF spectral library. The library is web-based, fully searchable and at moment includes spectra of 150 compounds from the most common chemical families. The fragmentation pattern of some chemical families is explained and a protocol for de novo identification has been provided in order to facilitate the identification of unknown compounds.
Conclusion:
A library for GC-APCI-QqToF is now publicly available online.
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