Controlling sources of preanalytical variability in doping samples: challenges and solutions

Area of Science:

• Sports Science
• Analytical Chemistry
• Biochemistry

Background:

• Antidoping testing is vital for sports integrity.
• Conventional lab testing focuses on analytical quality.
• Risks from unsuitable doping samples are often overlooked.

Purpose of the Study:

• To provide an overview of challenges in preanalytical management of doping samples.
• To discuss potential solutions to mitigate preanalytical variable impacts.
• To highlight the importance of sample quality in antidoping.

Main Methods:

• Review of preanalytical variables affecting doping sample quality.
• Discussion of challenges in sample collection, handling, transportation, and storage.
• Personal overview of current issues and proposed solutions.

Main Results:

• Key preanalytical variables include biological variability, collection issues, hemolysis, and degradation.
• Inappropriate transportation and storage conditions degrade sample quality.
• Overlooking preanalytical factors compromises antidoping test reliability.

Conclusions:

• Strict adherence to preanalytical criteria is essential for reliable doping control.
• Addressing preanalytical variables is critical to prevent negative impacts on sample quality and test results.
• Improved preanalytical management enhances the effectiveness of antidoping efforts.