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Updated: Aug 12, 2026

Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release
Published on: October 6, 2018
Release of corticotrophin releasing factor and other hypophysiotropic substances from isolated nerve-endings
Abstract:
Synaptosomes isolated from the sheep and the rat hypothalamus contain corticotrophin releasing factor, vasopressin, prolactin-release inhibiting factor and probably all of the substances which participate in the regulation of adenohypophysial function. Electrical field stimulation or depolarizing concentrations (55 mmol/l) of potassium ions cause a release of these factors from the incubated nerve-endings in a calcium-dependent manner. It is suggested that synaptosomes may provide a valuable approach to the study of mechanisms involved in hypothalamic neurosecretion in vitro.
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