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Proteomics analysis of human pericardial fluid
Fei Xiang1, Xuejiang Guo, Wen Chen
1Department of Thoracic and Cardiovascular Surgery, Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing, P. R. China.
Proteomics
|June 26, 2013
Summary
This study presents the first comprehensive proteomic analysis of human pericardial fluid (PF), identifying 1007 proteins. This research establishes a foundation for using PF in cardiovascular research.
Area of Science:
- Cardiovascular Biology
- Proteomics
- Biochemistry
Background:
- Pericardial fluid (PF) serves as a crucial biochemical indicator of cardiac health.
- Previous studies have not extensively analyzed the PF proteome.
- Understanding the PF proteome can offer new insights into cardiovascular conditions.
Purpose of the Study:
- To conduct the first in-depth, comprehensive proteomic analysis of human pericardial fluid.
- To identify a wide range of proteins present in PF, including low-abundant ones.
- To establish a foundational dataset for future cardiovascular research utilizing PF.
Main Methods:
- Utilized an SDS-PAGE-LC-MS/MS platform for proteomic analysis.
- Applied depletion techniques to enhance the coverage of low-abundant proteins.
- Identified nonredundant proteins based on a minimum of two identified peptides.
Main Results:
- Successfully identified 1007 nonredundant proteins in human pericardial fluid.
- Achieved broad coverage of the PF proteome, including previously undetected low-abundance proteins.
- The dataset has been deposited in the ProteomeXchange repository (identifier PXD000194).
Conclusions:
- This study provides the first comprehensive human pericardial fluid proteome dataset.
- The findings offer a valuable resource for advancing cardiovascular research.
- Pericardial fluid analysis holds significant potential for diagnosing and understanding heart diseases.
