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Selaginellatamariscina attenuates metastasis via Akt pathways in oral cancer cells

Jia-Sin Yang1, Chiao-Wen Lin, Chung-Han Hsin

  • 1Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.

Plos One
|June 27, 2013
PubMed
Abstract

Insights

Selaginella tamariscina inhibits oral cancer cell invasion by down-regulating matrix metalloproteinases (MMP-2 and MMP-9). This oriental herb may serve as an adjuvant therapy to prevent oral cancer metastasis.

Area of Science:

  • Oncology
  • Pharmacology
  • Molecular Biology

Background:

  • Selaginella tamariscina, an oriental medicinal herb, shows therapeutic potential for various diseases.
  • Human oral squamous-cell carcinoma (OSCC) is a significant health concern.
  • Investigating the anti-cancer mechanisms of S. tamariscina is crucial for developing new treatments.

Purpose of the Study:

  • To elucidate the mechanisms by which Selaginella tamariscina inhibits the invasiveness of human oral squamous-cell carcinoma (OSCC) HSC-3 cells.
  • To evaluate the anti-metastatic potential of S. tamariscina extracts.

Main Methods:

  • Cell migration and invasion assays were performed.
  • Matrix metalloproteinases (MMP)-2 and MMP-9 activity and expression were analyzed.
  • Quantitative real-time PCR, reporter assays, and chromatin immunoprecipitation (ChIP) were utilized to assess transcriptional regulation.
  • Western blotting and kinase activity assays were employed to study signaling pathways.

Main Results:

  • Selaginella tamariscina dose-dependently inhibited HSC-3 cell migration and invasion.
  • The herb downregulated the activity and expression of MMP-2 and MMP-9 at the transcriptional level.
  • Inhibition involved decreased binding of transcription factors (CREB, AP-1, SP-1) to MMP promoters and reduced Akt activation.

Conclusions:

  • Selaginella tamariscina exhibits potent anti-metastatic properties against oral cancer cells.
  • The downregulation of MMP-2 and MMP-9 is a key mechanism of action.
  • Selaginella tamariscina shows promise as an adjuvant therapeutic agent for oral cancer prevention.

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